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use of variables based on aggregated data that could hardly be used by other methodologies. The varroa mite is one of the main causes of honey bee mortality. An important mechanism by which honey bees increase their resistance against this mite is the expression of suppressed mite reproduction. This trait describes the physiological inability of mites to produce viable offspring and was found associated with eight genomic variants in previous research. This paper presents the development and validation of high-throughput qPCR assays wi
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