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DA oxidation and PDA formation were followed over time through the SERS band profile at pH 7, 9 and 12. We found that in situ PDA formation started after 50 min of laser irradiation of DA at pH 7, while DA was quickly oxidized at pH 9 and 12. Here, we present a detailed SERS band analysis of PDA, which sheds light on the molecular steps in the pathway formation of the PDA structure. Spectroscopic analysis and characterization revealed that a long laser exposure time led to the formation of stable PDA complexes with AgNPs, which allowed us to propose a novel
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