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in age as well the high proportion of time that poorly adherent adolescents spend in this state. © 2020 The Authors. Journal of the International AIDS Society published by John Wiley Sons Ltd on behalf of the International AIDS Society.De novo transcriptome assembly is a powerful tool, widely used over the last decade for making evolutionary inferences. However, it relies on two implicit assumptions that the assembled transcriptome is an unbiased representation of the underlying expressed transcriptome, and that expression estimate
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