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Superior intermediate epithelial thickness was the best marker for diagnosing DED using OCT (binormal estimated area under ROC 0.87; best cut-off value 50 µm thickness). The difference between the inferior and superior peripheral zones was the best marker for grading the severity of DED (analysis of variance, p=0.009). A multivariate approach identified other significant covariables which were integrated into a multivariate model to improve the sensitivity (86.4%) and specificity (91.7%) of this innovative diagnostic method. Including
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