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The methods are optimized for T. reesei but can also be applied for such transformation-resilient species as T. harzianum and T. guizhouense, which are putative upcoming alternatives for T. reesei in this field. The protocols are simple, do not require extensive training or special equipment, and can be further adjusted for T. reesei mutants with particular properties.Sustainable biofuel sources require the new sources of biofuel crops that can be developed into scalable plantation to meet the growing energy demands. Diverse supply sourc

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