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These matrix effects were traced back to inorganic anions and cations, which eluted over a significant fraction of the chromatographic run time with this setup. Hence, it was concluded that inorganic ions are a main cause for matrix effects in the analysis of mobile chemicals utilizing HILIC. Graphical abstract.Cell population heterogeneities and their changes in mammalian cell culture processes are still not well characterized. In this study, the formation and dynamics of cell population heterogeneities were investigated with flow cyto
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