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19 ± 0.02 g/L and 0.537 ± 0.053 for tris-grown culture, respectively. The pH 7.0-7.5 was determined as an optimum, whereas pH 6.5-7.0 and 8.0-8.5 were found to affect the growth and induce palmelloidy. The OD and AFDW of culture grown in NH4HCO3 were found equivalent to a standard nitrogen source (NH4Cl), whereas culture shown poor growth in urea. Based on these data, NH4HCO3 media recipe and the optimized cultivation parameters were selected for photoautotrophic cultivation of Chlamydomonas in greenhouse open ponds. To assess the readab
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