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MSIsensor-ct is freely available at https//github.com/niu-lab/MSIsensor-ct. Supplementary data are available at Briefings in Bioinformatics online. Supplementary data are available at Briefings in Bioinformatics online.Lungfishes belong to lobe-fined fish (Sarcopterygii) that, in the Devonian period, 'conquered' the land and ultimately gave rise to all land vertebrates, including humans1-3. Here we determine the chromosome-quality genome of the Australian lungfish (Neoceratodus forsteri), which is known to have the largest genome of any