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The correlation results indicated that no significant correlation was observed between the TC concentration and gene abundances of TC-ARGs and their expression levels. Nevertheless, the gene abundances of tetC and tetO were significantly correlated with their expression levels, thus indicating that they can be used to evaluate and assess expression levels to a certain extent.In order to explore the conjugation of genes encoding extended-spectrum β-lactamase (ESBL), ESBL-expressing P. aeruginosa and E.coli strains isolated from the wast