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This study investigated the tolerance of plants to vanadium (Ⅴ). The hydroponic method was employed to evaluate the absorption, transport, content, and subcellular distribution of vanadium in the polysaccharide fraction of corn seedlings cell wall under different concentrations of vanadium stress. Results showed that (a) vanadium was mainly concentrated in the roots of the corn seedlings, and only trace amounts were transported to the leaves; (b) in terms of its subcellular distribution, vanadium was mainly enriched in cell wall regions