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Our findings indicate a role for E2 in expression regulation of factors related to CL function and a novel potential for E2 to regulate DNA methylation as putative physiological mechanisms controlling luteal gene expression.In pursuit of precision livestock farming, the real-time measurement for heat strain-related data has been more and more valued. Efforts have been made recently to use more sensitive physiological indicators with the hope to better inform decision-making in heat abatement in dairy farms. To get an insight into the earl