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The hydrolytic mechanism of the enzyme was elucidated using capillary zone electrophoresis and molecular docking calculations. Abn93T has an ability to produce (in synergism with arabinofuranosidases) arabinose and arabinobiose from sugar beet arabinan, which can be explored as fermentable sugars and prebiotics. KEY POINTS • Thermophilic exo-arabinanase from family GH93 • Molecular basis of arabinan depolymerization. Nowadays, E-cigarette use shows a significant increase among adults and youth in many countries, although there is still a

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