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And Spag6 protein is distributed in different genotypes of mouse hair cells; Spag-/- mice showed otitis media. The expression of Prestin mRNA and protein in Spag-/- mice was significantly higher than that in Spag+/+ mice (P less then 0.01). The expression of Pgrn gene in Spag+/+ mice was significantly higher than that in Spag-/- mice (P less then 0.05). It indicates that the loss of Spag6 gene would lead to the decline of hearing sense in mice. It is likely that the Spag6 gene could affect hearing by regulating the expression of Prestin
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