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19F NMR spectroscopy provides the power to quantitatively analyze solitary species in complex solutions it is often restricted to the small susceptibility built-in to NMR. 4R- and 4S-Perfluoro-tert-buyl hydroxyproline contain 9 equivalent fluorines, in amino acids with powerful conformational preferences. In order to test the capability to use these amino acids as delicate probes of protein improvements, the perfluoro-tert-buyl hydroxyprolines had been included
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