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We provide one step by step protocol to steer through the technique and also to emphasize prospective problems and technical troubles. The protocol covers the next aspects experimental options and required material, anesthesia of this mouse, dissection regarding the cremaster muscle mass along with tracheal and carotid cannulation, IVM tracks and traditional analysis. Data platforms like adherent leukocytes, moving flux (RF) and rolling flux fraction (RFF) are explained in

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