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After well-informed consent, we obtained potential blood samples from a cohort of 12 melanoma clients with tissue-confirmed BRAF V600E mutation. Each blood specimen was processed instantly post collection using two posted standard protocols in synchronous selecting for EVs and platelets, respectively. The RNA of each and every fraction had been analysed by an extremely sensitive ARMS RT-qPCR enabling the measurement for the mutant allele fraction (%MAF) of BRAF V600E down to
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