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05) and nitric oxide (NO, P less then 0.01) concentrations were higher. Coccygeal vein concentrations of Cys (P less then 0.01), Pro (P less then 0.01), Tyr (P = 0.05), most essential AA except Thr and His (P less then 0.01), total essential AA (P less then 0.01), and total AA (P less then 0.01) were higher in the NCG-cows than in the CON-cows. The arterial supply of all AA was greater in the NCG-cows than in the CON-cows. The NCG-cows had higher mammary plasma flow of AA (P = 0.04) and clearance rate of Cys (P less then 0.01), Pro (P le