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Introduction The aim of this study was to evaluate the influence of different intracanal materials and the metallic artifact reduction tool (MAR) of cone-beam computed tomography (CBCT) on the detection of the second mesiobuccal (MB2) canal in maxillary first molars. Methods Forty maxillary first molars were selected, such that half of them had the MB2 canal in the mesiobuccal root confirmed by micro-computed tomography. All teeth were instrumented, except for the MB2 canal, and filled with gutta-percha, which was passively inserted up