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Here, we concisely summarize effective examples in exploiting E. coli for assembly-line polyketide biosynthesis as well as offer types of brand-new challenges for which this approach is primed to tackle.Fatty alcohols are essential industrial oleochemicals with broad applications and an ever growing market. Here, we sought to engineer Yarrowia lipolytica to act as a renewable supply of fatty alcohols (specifically hexadecanol, heptadecanol, octadecanol, and oleyl alcohol) straight from sugar. Through screen