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78-69.06%, and dark sulfide oxidation decreased by 76.36-94.29%. N application had little effect on fungal community structure and function. In general, short-term N application directly affected bacterial community structure and indirectly affected bacterial community structure and function through available potassium, while soil organic carbon was an important factor affecting fungal community structure and function.Bacterial antibiotic resistance sets a great challenge to human health. It seems that the bacteria can spontaneously evo