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To evaluate relative cytotoxicity of antibiotics to normal canine joint tissues in vitro. Experimental in vitro study. Chondrocytes and synoviocytes (three dogs); cartilage explants (three dogs); six dogs total. Chondrocytes and synoviocytes from normal femoropatellar joints of three dogs were plated on 24-well plates (50 000 cells/cm , triplicate, 48 hours) and exposed to antibiotics (ampicillin sulbactam, vancomycin, cefazolin, ceftazidime, amikacin, enrofloxacin; 0.39-25 mg/mL, 24 hours). Viability was assessed by using trypan blue d