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EDS analysis further confirmed the filler minerals in the sample paper with a trace amount of lead, which decreased upon bleaching the paper. The structure and properties of the sample and the recycled papers were quite similar, inferring that waste papers can be recycled again and different products from low to higher grade papers can be fabricated.Humoral practice is a fundamental natural biological phenomenon in earthworm defensive system which protects them from infectious bacteria and irritating agents by different mechanisms. The