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Four fungal and one bacterial isolates were isolated from a liquid waste sample of Nuclear Material Authority. Those dried biomasses were screened for uranium (U) and thorium (Th) adsorption efficiency where the most potent isolate was identified according to sequence similarities and phylogenetic analysis as Aspergillus niger LBM 134. Using U or Th synthetic solutions many factors were investigated for controlling the biosorption process to conduct the optimum process conditions (the solution pH, contact time, elemental initial concentrat