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This article is safeguarded by copyright. All liberties reserved.The synthesis of Asn-tRNAAsn in many prokaryotes makes use of an indirect aminoacylation pathway involving a non-discriminating aspartyl-synthetase (ND-AspRS) and a glutamine amidotransferase (GatCA. It was recently implicated as an adaptive mistranslation apparatus for antimicrobial opposition in Mycobacterium tuberculosis, nonetheless it remains poorly recognized. We report an accessible fluid chromatography-
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