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Additionally, we found an accumulation of branched-chain α-keto acids (BCKAs), the first degradation products resulting from transamination of BCAAs. In wild-type plants, pyruvate, glycine and BCKAs are all metabolized through decarboxylation by mitochondrial lipoyl cofactor-dependent dehydrogenase complexes. These enzyme complexes are very abundant, comprising a major sink for lipoyl cofactor. Because biosynthesis of lipoyl cofactor depends on continuous Fe-S cluster supply to lipoyl synthase, this could explain why lipoyl cofactor-depend

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