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GEWIS identified multiple significant genes-long-term antibiotic use during early life interaction effects, such as ANK3 (rs773585997, p value = 1.78 × 10-8) for anxiety and STRN (rs140049205, p value = 1.88 × 10-8) for depression. GO enrichment analysis detected six GO terms enriched in the identified genes interacting with long-term antibiotic use during early life for anxiety, such as GO0030425~dendrite (p value = 3.41 × 10-2) and GO0005886~plasma membrane (p value = 3.64 × 10-3). Our study results suggest the impact of long-term antib