https://ripkinasesignaling.com..../index.php/the-actua
However, in some cases, fusion of cumbersome affinity tags might present a substantial restriction during these experiments, particularly if the areas in close proximity of tags take part in protein-protein interactions. Right here, we present a step-by-step protocol for an alternative method which involves reversible biotinylation of purified proteins utilizing a straightforward chemical-conjugation of cleavable biotin moiety. Biotinylated proteins could be right use
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