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al resuppression illustrates the profound impact of PDR on treatment outcomes. We sought to determine vaccine antibody titres and the prevalence of hepatitis B surface antigen (HBsAg) in both HIV-positive and HIV-negative infants born to HIV-positive mothers in Botswana. This was a retrospective cross-sectional study using 449 archived dried blood spot samples from both HIV-positive and HIV-negative infants collected between 2016 and 2018. We screened dried blood spot samples for HBsAg and determined hepatitis B surface antibody titres.