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Plant size influences plant responses to combined environmental factors under climate change. However, their roles in plant ecophysiological responses are not fully understood. Two rapidly growing Leguminosae species (Robinia pseudoacacia and Amorpha fruticosa) were used to examine plant responses to combined drought and defoliation treatments (two levels of both treatments). Both 1.5 month-old seedlings and 3 month-old seedlings were grown in a greenhouse, and seedling growth, leaf gas exchanges, stem hydraulics, and concentrations of