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Bacterial viability and biofilm development after exposure of micro-organisms towards the hydrogels were used as effectiveness indicators. Live/Dead assay ended up being used to measure the amount of compromised micro-organisms utilizing a confocal laser scanning microscope. The results show that the antibacterial hydrogel surely could kill more or less 99% regarding the revealed micro-organisms after 3 h of visibility. In addition, NIH/3T3 fibroblasts were utilized to study