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Neonicotinoid insecticides (NEOs) have attracted particular attention in recent years due to their wide occurrence and potential impacts on the ecosystem and human health. This study aimed to compare the composition and level of NEOs in soils of different land use types. Two rounds of sampling were performed in Tianjin, China, with 158 soil samples in fall and 61 soil samples in spring collected from five types of land, i.e., greenhouse, orchard, farm, park and residential area. The concentrations of eight NEOs, i.e., imidacloprid (IMI),