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Moist condition of both 3D printed heart/porcine heart surface and applicator at transplantation yielded highest successful rate (100%, p=0.0197). For both endoscopic transplantation sites, MSC sheets were successfully deployed. The procedure duration was 157±23s for anterior wall and 123±13s for the lateral wall in average, respectively. We developed a novel prototype of endoscopic cell sheet delivery device for minimally-invasive cardiac regenerative therapy utilizing a 3D printed simulator. The commercialization of the prot