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Because of the translucency for the Daphnia human body additionally the application of fluorescent probes in mobile staining, different intoxicated parts could be visualized. In this study, a double-staining technique making use of two fluorescent dyes, Calcein AM (cell-permeant dye) and Propidium Iodide (cell-impermeant dye), was done on Daphnia magna subjected to six pathogens Salmonella spp. (four strains) and Shigella spp. (two strain
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