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It did not, which indicates that the influence on group estradiol levels may be specific to cues from the queen. These queen cues may be behavioral in nature, as we found that queens were less aggressive during pregnancy, which prior work has suggested may relax reproductive suppression of subordinates. Yet levels of queen aggression alone were not associated, or were weakly associated, with their colony's estradiol levels, though our sample size examining this particular relationship was low. This is suggestive that addition

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