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The pollution of karst aquifers by acid mine drainage (AMD) waters is increasing. Major and minor ions (Ca2+, Mg2+, HCO3-, SO42-, F-, and Fe), stable sulfur and oxygen isotopes of dissolved sulfates (δ34SSO4 and δ18OSO4) and oxygen isotope of water (δ18OH2O), were analyzed in rainwater, surface water, groundwater, and AMD water sampled from the Babu subterranean stream watershed, in Southwest China. The principal aim of this study was to explore the impact of AMD waters on the evolution of karst aquifers. Based on hydrogeochemistry an