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The use of the trisiloxane Si3/iSi3 segment primarily led to the formation of columnar (Col) phases. Consideration of the chemical composition revealed that the delicate balance between the three molecular moieties, siloxane terminal, alkyl spacer, and aromatic core part, is critical for the Cub phase formation, and it can be summarized as the threshold weight fraction of the alkyl spacer in the three moieties being 0.284 or larger, which derives an anti-spindle shape favorable for the formation of the Ia3d-Cub phase.In an open microfluidi