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9%±0.3% for the forward calculated patient plans at 3%/3mm gamma tests. Dose prediction using the trained network was very fast at approximately 0.9 seconds for the input generation and 0.6 seconds for single GPU inference per segment and 1 minute per patient in total. The overall performance of this dose calculation framework in terms of both accuracy and inference speed, makes it compelling for online adaptive workflows where fast segment dose calculations are needed. Creative Commons Attribution license.Intravital imaging of brain