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In this research, the extracellular polymeric substance (EPS) extracted from Rahnella sp. LRP3 was discovered because of the potential of immobilizing Cu-polluted in soil. The EPS could connect to Cu (II) through functional groups (polysaccharides, amide, proteins, and carboxyl groups), which further developed to the porous sphere with a diameter of 20 μm. Besides, EPS could induce the forming of Cu5(PO4)2(OH)4 crystal because of t