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Daphnia magna signifies an excellent aquatic design of these ecological studies, due to its similarities with vertebrates in several neurotransmitters and relevant gene paths and due to the wide application in ecotoxicological researches. Through this study, an exact and sensible method of evaluation of 17 neurotransmitters and associated precursors and metabolites was developed. The technique had been validated when it comes to sensitiveness, reproducibility, accuracy, and accuracy,
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