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In addition, Drosophila C virus (DCV), a well-characterized D. melanogaster virus, was utilized as an optimistic control, because it features formerly shown a locomotor defect in contaminated flies. Shares infected with NV (NV+) and DCV (DCV+) and virus-free (NV-/DCV-) stocks had been set up. Over a 3-year duration, about 2,500 virgin female flies had been tested for geotaxis and longevity utilizing Kaplan-Meier analyses, plus the Cox Proportional Haz