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Analysis of allele frequencies of short tandem repeat (STR) loci in ethnically diverse populations is essential for forensic reference database construction and studies on population genetics. To analyse genetic polymorphisms of 22 autosomal STR loci in the Serbian population and to compare them with previously published data from some European and Turkish populations. The study was conducted among 983 unrelated individuals from Serbia. Genotyping was performed using the PowerPlex Fusion amplification kit. Allele frequencies and forens