https://kaempferidechemical.co....m/making-and-repair-
Here we report the introduction of a novel transposon, MycoTetOP 2, to help the identification of such genes by direct transposon mutagenesis. This mariner-based transposon includes nested anhydrotetracycline (ATc)-inducible promoters to push transcription outward from each of its finishes. In inclusion, it offers the Escherichia coli R6Kγ source to facilitate the identification of insertion sites. MycoTetOP 2 had been put into a shuttle plasmid with a temperature-sensitive DNA replication o
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