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The activities of biohydrogen production through active/passive saccharification and photo-fermentation were compared. The consequences of preliminary pH, substrate concentration, and cellulase loading on biohydrogen production from alfalfa by photosynthetic germs HAU-M1 were presented. It was unearthed that the utmost hydrogen yield of 55.81 mL/g ended up being accomplished at preliminary pH of 6.90, substrate concentration of 31.23 g/mL, and
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