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Background Infection prevention and control measures are critical for the prevention of the spread of COVID-19. Aim In this study, we aimed to measure and evaluate the level of awareness and knowledge of the prevention, symptoms, and transmission control of COVID-19 before and after quarantine among the residents of Rabigh city and adjacent villages in Saudi Arabia. Methods A cross-sectional online survey was conducted in two stages the first stage took place before quarantine and the second stage took place after quarantine. The survey wa