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Epilepsy is a chronic neurodegenerative disease that has seriously threatened human health. Accumulating evidence reveals that the pathological progression of epilepsy is closely related to peroxynitrite (ONOO-). Unfortunately, understanding the physiological roles of ONOO- in epilepsy is still challenging due to the lack of powerful imaging probes for the determination of the level of fluctuations of ONOO- in the epileptic brain. Herein, a near-infrared (NIR) two-photon (TP) fluorescent probe [dicyanomethylene-4H-pyran (DCM)-ONOO] is pr
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