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5 and 1 kg LS groups (P less then 0.001). The total aerobic bacterial load of the eggshell was the lowest in the 1 kg LS group (4.7 log10 cfu/mL). The level of aspartate amino transferase and alanine amino transferase showed an increment in both the CONT and 2 kg LS groups (P less then 0.001). The high level of LS (2 kg per ton of feed) caused a decline in the levels of IgY, IgA and IgM, when compared to the 0.5 and 1 kg LS groups (P less then 0.001). Laying hens in 0.5 and 1 kg LS groups had longer villus height (1335.9 μm) in the jej
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