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A study was conducted to determine the optimum dosage of the exogenous cholesterol-loaded-cyclodextrins (CLC) to get maximum cryoprotection for bubaline spermatozoa. In the present study, 120×106 spermatozoa were incubated in 2, 3 and 4 mg of CLC as grouped as Gr II, III and IV, respectively and sperm progressive motility, intracellular Ca2+ , capacitation status by protein tyrosine phosphorylation (PTP) assay and zona binding percent (ZBP) and cleavage rate (CR) of the cryopreserved buffalo spermatozoa by in-vitro fertility assay were a
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