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A subset of the toxicants were then reexamined in hypoosmotic and hyperosmotic media. Copper toxicity decreased in hyperosmotic medium, and nickel toxicity increased in hypoosmotic and hyperosmotic media. Linear alkylbenzene sulfonate toxicity was not affected by the medium osmolality. Overall, RTgill-W1 cells have shown potential for applications in measuring metal, metalloids, ammonia, and water-soluble organic chemicals in acute WET tests, as well as complementing current toxicity identification and reduction evaluation strategies. In the present study,
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