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The IgG format with bivalent binding websites features a modular structure with different biological roles, i.e., effector and binding functions, in numerous domain names. We demonstrated the repair of an IgG-like domain framework in vitro by protein ligation utilizing necessary protein trans-splicing. We produced numerous binding domains to restore the binding domain of IgG from Escherichia coli in addition to Fc domain of individual IgG from Brevibacillus choshinensis as split-intein fusions. We rev

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