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This indicated that DESI-MSI could accurately visualize αLA and OPDA, while DESI-MS/MSI was necessary to visualize OPC-80. The results demonstrated that free αLA and OPC-80 were abundant in the radicle and seed coat, while free OPDA was accumulated in the seed coat. Interestingly, the localization pattern of OPDA was similar to that of JA. In addition, compared to the concentrations of OPDA, the concentration of OPC-80 was lower in the seed coat and higher in the radicle. These results suggest that OPDA and/or JA play a biological role mai
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